Degradation of nuclear proteins by autophagy
Group Leader, Scientific Coordinator
Sascha Martens obtained his Diploma and Doctoral degrees from the University of Cologne in Germany. For his postdoctoral training he moved to the MRC Laboratory of Molecular Biology in Cambridge, UK. In 2009 he established his independent laboratory at the Max Perutz Labs Vienna.
- Institute Max Perutz Labs, University of Vienna
- Phone +43 1 4277 52876
- Mail firstname.lastname@example.org
Projects within consortium
Autophagy is a conserved cytoplasmic degradation pathway for the degradation of harmful substances within lysosomes. This is achieved by the encapsulation of this cargo material by double membrane organelles – the autophagosomes. Among the many cargoes that are degraded by this pathway are damaged organelles, intercellular pathogens and aggregated proteins. Autophagy thereby protects us from various diseases including cancer and neurodegeneration.
In the cytoplasm autophagy acts together with the ubiquitin-proteasome system (UPS) in the degradation of ubiquitinated proteins. For autophagic degradation, these proteins are bound by cargo receptors, which facilitate the phase separation of these proteins into larger condensates. Subsequently, they recruit the autophagy machinery and link the condensates to the autophagosomal membrane. Finally, these autophagosomes fuse with lysosomes, wherein the ubiquitinated proteins are degraded.
While in the cytoplasm the process of protein degradation can rely on autophagy as backup system for the ubiquitin-proteasome system, it is unclear how the nucleoplasm copes with situations under which the UPS is overwhelmed. Since autophagosome formation and lysosomal degradation are confined to the cytoplasm, the entire process of autophagy cannot occur in the nucleoplasm. We aim to understand to which extent nuclear proteins are exported into the cytoplasm for autophagic degradation. We are particularly interested to study this process in long-lived postmitotic cells, in which the nuclear envelope does not periodically break down.
Targeted Protein Degradation related publications by Group Martens
- 2021 Reconstitution defines the roles of p62, NBR1 and TAX1BP1 in ubiquitin condensate formation and autophagy initiation Nat. Commun. Go to publication →
- 2020 Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation Science Go to publication →
- 2020 A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress eLife Go to publication →
- 2020 A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy Journal of Cell Biology Go to publication →
- 2019 FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates Mol. Cell Go to publication →
- 2018 p62 filaments capture and present ubiquitinated cargos for autophagy EMBO J. Go to publication →
- 2016 Mechanism of cargo-directed Atg8 conjugation during selective autophagy eLife Go to publication →
- 2015 Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy eLife Go to publication →
- 2014 Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane–cargo apposition during selective autophagy Nat. Cell Biol. Go to publication →