Targeted Protein Degradation

Publications

Selected Publications

  • 2024 PITHD1: An Endogenous Inhibitor of the 26S Proteasome During Cellular Dormancy bioRxiv Amann SJ, Dong K, Roehsner J, Krall D, Grishkovskaya I, Kotisch H, Schleiffer A, Roitinger E, Pauli A, Martin A, Haselbach D Go to publication →
  • 2024 Seipin governs phosphatidic acid homeostasis at the inner nuclear membrane Nature Communications Romanauska A, Stankunas E, Schuldiner M, Köhler A Go to publication →
  • 2024 A multivalent adaptor mechanism drives the nuclear import of proteasomes bioRxiv Brunner HL, Kalis RW, Grundmann L, Hodáková Z, Koskova Z, Grishkovskaya I, de Almeida M, Hinterndorfer M, Höfflin S, Andersch F, Kotisch H, Dickmanns A, Cuylen-Häring S, Zuber J, Haselbach D Go to publication →
  • 2024 Recruitment of autophagy initiator TAX1BP1 advances aggrephagy from cargo collection to sequestration EMBO Journal Bauer B, Idinger J, Schuschnig M, Ferrari L, Martens S Go to publication →
  • 2024 IGF2BP1 phosphorylation in the disordered linkers regulates ribonucleoprotein condensate formation and RNA metabolism Nature Communications Hornegger H, Anisimova AS, Muratovic A, Bourgeois B, Spinetti E, Niedermoser I, Covino R, Madl T, Karagöz GE Go to publication →
  • 2024 Cross-species interactome analysis uncovers a conserved selective autophagy mechanism for protein quality control in plants bioRxiv Sánchez de Medina Hernández V, Nava García MM, Clavel M, Papareddy RK, Andreev VI, Mathur V, Mohseni A, García-León M, Gao P, de la Concepción JC, Picchianti L, Grujic N, Kobylinska R, Abdrakhmanov A, Duvergé H, Anand G, Leibrock G, Bianchi A, Raffeiner M, Crawford TC, Argirò L, Matuszkiewicz M, Wun CL, Kanne JV, Meinhart A, Roitinger E, Bäurle I, Kang BH, Petersen M, Üstün S, Kulathu Y, Clausen T, Ramundo S, Dagdas Y Go to publication →
  • 2024 Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering EMBO Journal Kettel P, Marosits L, Spinetti E, Rechberger M, Giannini C, Radler P, Niedermoser I, Fischer I, Versteeg GA, Loose M, Covino R, Karagöz GE Go to publication →
  • 2024 Inhibitor-induced supercharging of kinase turnover via endogenous proteolytic circuits bioRxiv Scholes NS, Bertoni M, Comajuncosa-Creus A, Kladnik K, Frommelt F, Hinterndorfer M, Razumkov H, Prokofeva P, Schwalm MP, Imrichova H, Barone E, Schätz C, Rukavina A, Koren A, Kubicek S, Knapp S, Gray MS, Superti-Furga G, Küster B, Aloy P, Winter GE Go to publication →
  • 2024 Monovalent Pseudo-Natural Product Degraders Supercharge the Native Degradation of IDO1 by KLHDC3 bioRxiv Hennes E, Lucas B, Scholes NS, Cheng XF, Scott DC, Bischoff M, Reich K, Gasper R, Luca M, Xu TT, Pulvermacher LM, Dötsch L, Imrichova H, Brause A, Naredla KS, Sievers S, Kumar K, Janning P, Gersch M, Murray PJ, Schulman BA, Winter GE, Ziegler S, Waldmann H Go to publication →
  • 2024 Alkylamine-tethered molecules recruit FBXO22 for targeted protein degradation Nature Communication Kagiou C, Cisneros JA, Farnung J, Liwocha J, Offensperger F, Dong K, Yang K, Tin G, Horstmann CS, Hinterndorfer M, Paulo JA, Scholes NS, Sanchez Avila J, Fellner M, Andersch F, Hannich JT, Zuber J, Kubicek S, Gygi SP, Schulman BA, Winter GE Go to publication →
  • 2024 Orpinolide disrupts a leukemic dependency on cholesterol transport by inhibiting OSBP Nature Chemical Biology Cigler M, Imrichova H, Frommelt F, Caramelle L, Depta L, Rukavina A, Kagiou C, Hannich JT, Mayor-Ruiz C, Superti-Furga G, Sievers S, Forrester A, Laraia L, Waldmann H, Winter GE Go to publication →
  • 2024 Tau fibrils evade autophagy by excessive p62 coating and TAX1BP1 exclusion Science Advances Ferrari L, Bauer B, Qiu Y, Schuschnig M, Klotz S, Anrather D, Juretschke T, Beli P, Gelpi E, Martens S Go to publication →
  • 2024 Guardian ubiquitin E3 ligases target cancer-associated APOBEC3 deaminases for degradation to promote human genome integrity bioRxiv Schwartz I, Budroni V, Meyenberg M, Hornegger H, Hacker K, Schwartz S, Hodakova Z, Grabarczyk DB, Ehrmann JF, Scinicariello S, Haselbach D, Menche J, Clausen T, Karagöz GE, Versteeg GA Go to publication →
  • 2024 A RabGAP negatively regulates plant autophagy and immune trafficking Current Biology Yuen ELH, Leary AY, Clavel M, Tumtas Y, Mohseni A, Zhao J, Picchianti L, Jamshidiha M, Pandey P, Duggan C, Cota E, Dagdas Y, Bozkurt TO Go to publication →
  • 2024 Endoplasmic reticulum: Monitoring and maintaining protein and membrane homeostasis in the endoplasmic reticulum by the unfolded protein response Int J Biochem Cell Biol Kettel P, Karagöz GE Go to publication →
  • 2024 TRIM52 is a primate-specific player in the DNA repair process under tight proteolytic control by a triad of giant E3 ligases bioRxiv Shulkina A, Hacker K, Ehrmann JF, Budroni V, Mandlbauer A, Bock J, Grabarczk DB, Cochella L, Clausen T, Versteeg GA Go to publication →
  • 2024 Metabolic enzymes moonlight as selective autophagy receptors to protect plants against viral-induced cellular damage bioRxiv Clavel M, Bianchi A, Kobylinska R, Groh R, Ma J, Papareddy RK, Grujic N, Picchianti L, Stewart E, Schutzbier M, Stejskal K, de la Concepcion JC, Sanchez de Medina Hernandez V, Yoichek Y, Clauw P, Gunis J, Durnberger G, Muelders JC, Grimm A, Sedivy A, Erhardt M, Vyboishchikov V, Gao P, Lechner E, Vantard E, Jez J, Roitinger E, Genschik P, Kang BG, Dagdas Y Go to publication →
  • 2024 Large-scale chemoproteomics expedites ligand discovery and predicts ligand behavior in cells Science Offensperger F, Tin G, Duran-Frigola M, Hahn E, Dobner S, Ende CWA, Strohbach JW, Rukavina A, Brennsteiner V, Ogilvie K, Marella N, Kladnik K, Ciuffa R, Majmudar JD, Field SD, Bensimon A, Ferrari L, Ferrada E, Ng A, Zhang Z, Degliesposti G, Boeszoermenyi A, Martens S, Stanton R, Müller AC, Hannich JT, Hepworth D, Superti-Furga G, Kubicek S, Schenone M, Winter GE Go to publication →
  • 2024 ATG8ylation of vacuolar membrane protects plants against cell wall damage bioRxiv Julian J, Gao P, Del Chiaro A, De La Concepcion JC, Armengot L, Somssich M, Duverge H, Clavel M, Grujic N, Kobylinska R, Polivka I, Besten M, Dank C, Korbei B, Bachmair A, Coll NS, Sprakel J, Dagdas Y. Go to publication →
  • 2024 BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition Plant Cell Zhang H, Rundle C, Winter N, Miricescu A, Mooney BC, Bachmair A, Graciet E, Theodoulou FL Go to publication →
  • 2024 Targeted protein degradation via intramolecular bivalent glues Nature Hsia O*, Hinterndorfer M*, Cowan AD*, Iso K, Ishida T, Sundaramoorthy R, Nakasone MA, Imrichova H, Schätz C, Rukavina A, Husnjak K, Wegner M, Correa-Sáez A, Craigon C, Casement R, Maniaci C, Testa A, Kaulich M, Dikic I, Winter GE†, Ciulli A† Go to publication →
  • 2023 Sequestration of translation initiation factors in p62 condensates Cell Reports Danieli A, Vucak G, Baccarini M, Martens S Go to publication →
  • 2023 Discovery of Molecular Glue Degraders via Isogenic Morphological Profiling ASC Chemical Biology Ng A, Offensperger F, Cisneros JA, Scholes NS, Malik M, Villanti L, Rukavina A, Ferrada E, Hannich JT, Koren A, Kubicek S, Superti-Furga G, Winter GE Go to publication →
  • 2023 Adult neural stem cells and neurogenesis are resilient to intermittent fasting EMBO Journal Gabarró-Solanas R, Davaatseren A, Kleifeld J, Kepčija T, Köcher T, Giralt A, Crespo-Enríquez I, Urbán N Go to publication →
  • 2023 Discovery of a Drug-like, Natural Product-Inspired DCAF11 Ligand Chemotype Nature Communication Xue G, Xie J, Hinterndorfer M, Cigler M, Dötsch L, Imrichova H, Lampe P, Cheng X, Adariani SR, Winter GE, Waldmann H Go to publication →
  • 2023 Optimized infrared photoactivatable ribonucleoside enhanced crosslinking and immunoprecipitation (IR-PAR-CLIP) protocol identifies novel IGF2BP3-interacting RNAs in colon cancer cells. RNA Anisimova AS, Karagöz GE Go to publication →
  • 2023 The potential of cross-linking mass spectrometry in the development of protein–protein interaction modulators Current Opinion in Structural Biology Ruwolt M, Piazza I, Liu F Go to publication →
  • 2023 SPOP targets the immune transcription factor IRF1 for proteasomal degradation eLife Schwartz I, Vunjak M, Budroni V, Cantoran García A, Mastrovito M, Soderholm A, Hinterndorfer M, de Almeida M, Hacker K, Wang J, Froussios K, Jude J, Decker T, Zuber J, Versteeg GA. Go to publication →
  • 2023 p62 and NBR1 functions are dispensable for aggrephagy in mouse ESCs and ESC-derived neurons Life Science Alliance Trapannone R, Romanov J, Martens S Go to publication →
  • 2023 Lipid saturation controls nuclear envelope function Nature Cell Biology Romanauska A, Köhler A Go to publication →
  • 2023 The membrane surface as a platform that organizes cellular and biochemical processes Developmental Cell Leonard TA, Loose M, Martens S Go to publication →
  • 2023 Analysis of higher plant N-degron pathway components and substrates via expression in S. cerevisiae Methods in Enzymology Böhm J, Winter N, Kozlic A, Telser T, Nehlin L, Bachmair A Go to publication →
  • 2023 Protein compactness and interaction valency define the architecture of a biomolecular condensate across scales eLife Polyansky AA, Gallego LD, Efremov RG, Köhler A, Zagrovic B Go to publication →
  • 2023 Cryo-EM structure of the chain-elongating E3 ubiquitin ligase UBR5 EMBO Journal Hodáková Z, Grishkovskaya I, Brunner HL, Bolhuis DL, Belačić K, Schleiffer A, Kotisch H, Brown NG, Haselbach D Go to publication →
  • 2023 Light-Cleavable Auxiliary for Diselenide–Selenoester Ligations of Peptides and Proteins Chemistry Europe Schrems M, Kravchuk AV, Niederacher G, Exler F, Bello C, Becker CFW. Go to publication →
  • 2023 Aggrephagy at a glance Journal of Cell Science Bauer B, Martens S, Ferrari L Go to publication →
  • 2023 Atg8 family proteins, LIR/AIM motifs and other interaction modes Autophagy Reports Rogov VV, Nezis IP, Tsapras P, Zhang H, Dagdas Y, Noda NN, Nakatogawa H, Wirth M, Mouilleron S, McEwan DG, Behrends C, Deretic V, Elazar Z, Tooze SA, Dikic I, Lamark T, Johansen T Go to publication →
  • 2023 HUWE1 controls tristetraprolin proteasomal degradation by regulating its phosphorylation eLife Scinicariello S, Söderholm A, Schäfer M, Shulkina A, Schwartz I, Hacker K, Gogova R, Kalis RW, Froussios K, Budroni V, Bestehorn A, Clausen T, Kovarik P, Zuber J, Versteeg GA. Go to publication →
  • 2023 Structural basis of how the BIRC6/SMAC complex regulates apoptosis and autophagy Science Ehrmann JF, Grabarczyk DB, Heinke M, Deszcz L, Kurzbauer R, Hudecz O, Shulkina A, Gogova R, Meinhart A, Versteeg GA, Clausen T Go to publication →
  • 2023 NDP52 acts as a redox sensor in PINK1/Parkin-mediated mitophagy EMBO Journal Kataura T, Otten EG, Rabanal-Ruiz Y, Adriaenssens E, Urselli F, Scialo F, Fan L, Smith GR, Dawson WM, Chen X, Yue WW, Bronowska AK, Carroll B, Martens S, Lazarou M, Korolchuk VI Go to publication →
  • 2023 Shuffled ATG8 interacting motifs form an ancestral bridge between UFMylation and C53-mediated autophagy EMBO Journal Picchianti L, Sánchez de Medina Hernández V, Zhan N, Irwin NAT, Stephani M, Hornegger H, Beveridge R, Sawa-Makarska J, Lendl T, Grujic N, Martens S, Richards TA, Clausen T, Ramundo S, Karagöz E, Dagdas Y Go to publication →
  • 2023 Advancing Targeted Protein Degradation via Multiomics Profiling and Artificial Intelligence Journal of American Chemical Society Duran-Frigola* M, Cigler M, and Winter GE* Go to publication →
  • 2023 A molecular network of conserved factors keeps ribosomes dormant in the egg Nature Leesch F, Lorenzo-Orts L, Pribitzer C, Grishkovskaya I, Roehsner J, Chugunova A, Matzinger M, Roitinger E, Belačić K, Kandolf S, Lin TY, Mechtler K, Meinhart A, Haselbach D, Pauli A Go to publication →
  • 2023 E3-Specific Degrader Discovery by Dynamic Tracing of Substrate Receptor Abundance Journal of American Chemical Society Hanzl A, Barone E, Bauer S, Yue H, Nowak RP, Hahn E, Pankevich EV, Koren A, Kubicek S, Fischer ES, Winter GE Go to publication →
  • 2022 Frozen in time: analyzing molecular dynamics with time-resolved cryo-EM Structure Amann SJ, Keihsler D, Bodrug T, Brown NG, Haselbach D Go to publication →
  • 2022 Orchestration of selective autophagy by cargo receptors Current Biology Adriaenssens E, Ferrari L, Martens S Go to publication →
  • 2023 SUMO Conjugation and SUMO Chain Formation by Plant Enzymes Methods in Molecular Biology Tomanov K, Julian J, Ziba I, Bachmair A Go to publication →
  • 2022 Characterization of ATG8-Family Interactors by Isothermal Titration Calorimetry Methods in Molecular Biology Picchianti L, Sedivy A, Dagdas Y Go to publication →
  • 2022 POLθ processes ssDNA gaps and promotes replication fork progression in BRCA1 deficient cells Cell Reports Schrempf A*, Bernardo S*, Arasa-Verge EA, Ramirez-Otero MA, Wilson J, Kirchhofer D, Timelthaler G, Ambros AM, Kaya A, Wieder M, Ecker GF, Winter GE, Costanzo V, Loizou JI Go to publication →
  • 2022 Site-selective photocatalytic functionalization of peptides and proteins at selenocysteine Nature Communications Dowman LJ, Kulkarni SS, Alegre-Requena JV, Giltrap AM, Norman AR, Sharma A, Gallegos LC, Mackay AS, Welegedara AP, Watson EE, van Raad D, Niederacher G, Huhmann S, Proschogo N, Patel K, Larance M, Becker CFW, Mackay JP, Lakhwani G, Huber T, Paton RS, Payne RJ Go to publication →
  • 2022 Cryo-EM structure of the chain-elongating E3 ligase UBR5 bioRxiv Hodáková Z, Grishkovskaya I, Brunner HL, Bolhuis DL, Belačić K, Schleiffer A, Kotisch H, Brown NG, Haselbach D Go to publication →
  • 2022 Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders Nature Chemical Biology Hanzl A, Casement R, Imrichova H, Hughes SJ, Barone E, Testa A, Bauer S, Wright J, Brand M, Ciulli A, Winter GE Go to publication →
  • 2022 Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole Journal of Cell Biology Zhao J, Bui MT, Ma J, Künzl F, Picchianti L, De La Concepcion JC, Chen Y, Petsangouraki S, Mohseni A, García-Leon M, Gomez MS, Giannini C, Gwennogan D, Kobylinska R, Clavel M, Schellmann S, Jaillais Y, Friml J, Kang BH, Dagdas Y Go to publication →
  • 2022 Lysosomal enzyme trafficking factor LYSET enables nutritional usage of extracellular proteins Science Pechincha C, Groessl S, Kalis R, de Almeida M, Zanotti A, Wittmann M, Schneider M, de Campos RP, Rieser S, Brandstetter M, Schleiffer A, Müller-Decker K, Helm D, Jabs S, Haselbach D, Lemberg MK, Zuber J, Palm W Go to publication →
  • 2022 BacPROTACs mediate targeted protein degradation in bacteria Cell Morreale FE, Kleine S, Leodolter J, Junker S, Hoi DM, Ovchinnikov S, Okun A, Kley J, Kurzbauer R, Junk L, Guha S, Podlesainski D, Kazmaier U, Boehmelt G, Weinstabl H, Rumpel K, Schmiedel VM, Hartl M, Haselbach D, Meinhart A, Kaiser M, Clausen T Go to publication →
  • 2021 McsB forms a gated kinase chamber to mark aberrant bacterial proteins for degradation eLife Hajdusits B, Suskiewicz MJ, Hundt N, Meinhart A, Kurzbauer R, Leodolter J, Kukura P, Clausen T Go to publication →
  • 2021 E3 ubiquitin ligase RNF213 employs a non-canonical zinc finger active site and is allosterically regulated by ATP bioRxiv Ahel J, Fletcher A, Grabarczyk DB, Roitinger E, Deszcz L, Lehner A, Virdee S, Clausen T Go to publication →
  • 2022 Transcriptome, metabolome and suppressor analysis reveal an essential role for the ubiquitin-proteasome system in seedling chloroplast development BMC Plant Biology Talloji P, Nehlin L, Hüttel B, Winter N, Černý M, Dufková H, Hamali B, Hanczaryk K, Novák J, Hermanns M, Drexler N, Eifler K, Schlaich N, Brzobohatý B, Bachmair A Go to publication →
  • 2022 Autophagy promotes programmed cell death and corpse clearance in specific cell types of the Arabidopsis root cap Current Biology Feng Q, De Rycke R, Dagdas Y, Nowack MK Go to publication →
  • 2022 Could a Different View of Quiescence Help Us Understand How Neurogenesis Is Regulated? Frontiers in Neuroscience Urbán N Go to publication →
  • 2022 Expressed Protein Selenoester Ligation Angewandte Chemie International Ed. Kulkarni SS, Watson EE, Maxwell JWC, Niederacher G, Johansen-Leete J, Huhmann S, Mukherjee S, Norman AR, Kriegesmann J, Becker CFW, Payne RJ Go to publication →
  • 2022 Cryo-EM structure of the plant 26S proteasome Plant Communications Kandolf S, Grishkovskaya I, Belačić K, Bolhuis DL, Amann S, Foster B, Imre R, Mechtler K, Schleiffer A, Tagare HD, Zhong ED, Meinhart A, Brown NG, Haselbach D Go to publication →
  • 2022 Molecular mechanisms of endomembrane trafficking in plants Plant Cell Aniento F, Sánchez de Medina Hernández V, Dagdas Y, Rojas-Pierce M, Russinova E Go to publication →
  • 2022 A yeast-based functional assay to study plant N-degron – N-recognin interactions Frontiers in Plant Science Kozlic A, Winter N, Telser T, Reimann J, Rose K, Nehlin L, Berckhan S, Sharma G, Dambire C, Boeckx T, Holdsworth MJ, Bachmair A Go to publication →
  • 2021 Stem cell quiescence: the challenging path to activation Development Noelia Urbán and Tom H. Cheung Go to publication →
  • 2021 AKIRIN2 controls the nuclear import of proteasomes in vertebrates Nature de Almeida M, Hinterndorfer M, Brunner H, Grishkovskaya I, Singh K, Schleiffer A, Jude J, Deswal S, Kalis R, Vunjak M, Lendl T, Imre R, Roitinger E, Neumann T, Kandolf S, Schutzbier M, Mechtler K, Versteeg GA, Haselbach D, Zuber J Go to publication →
  • 2021 Decomposing the mutational landscape of cancer genomes with RepairSig Cell Systems Bernardo S, Meyenberg M and Loizou JI Go to publication →
  • 2021 Wnt/beta-catenin signalling is dispensable for adult neural stem cell homeostasis and activation Development Austin SHL, Gabarró-Solanas R, Rigo P, Paun O, Harris L, Guillemot F, Urbán N Go to publication →
  • 2021 Proteasome and selective autophagy: Brothers-in-arms for organelle quality control Current Opinion in Plant Biology Clavel M, Dagdas Y Go to publication →
  • 2021 Reconstitution defines the roles of p62, NBR1 and TAX1BP1 in ubiquitin condensate formation and autophagy initiation Nature Communications Turco E, Savova A, Gere F, Ferrari L, Romanov J, Schuschnig M, Martens S Go to publication →
  • 2021 An oomycete effector subverts host vesicle trafficking to channel starvation-induced autophagy to the pathogen interface eLife Pandey P, Leary AY, Tumtas Y, Savage Z, Dagvadorj B, Duggan C, Yuen EL, Sanguankiattichai N, Tan E, Khandare V, Connerton AJ, Yunusov T, Madalinski M, Mirkin FG, Schornack S, Dagdas Y, Kamoun S, Bozkurt TO Go to publication →
  • 2021 The rise of proteome‐wide biophysics Molecular Systems Biology Mateus A, Savitski MM, Piazza I Go to publication →
  • 2021 HUWE1 employs a giant substrate-binding ring to feed and regulate its HECT E3 domain Nature Chemical Biology Grabarczyk DB, Petrova OA, Deszcz L, Kurzbauer R, Murphy P, Ahel J, Vogel A, Gogova R, Faas V, Kordic D, Schleiffer A, Meinhart A, Imre R, Lehner A, Neuhold J, Bader G, Stolt-Bergner P, Böttcher J, Wolkerstorfer B, Fischer G, Grishkovskaya I, Haselbach D, Kessler D, Clausen T Go to publication →
  • 2021 The linear ubiquitin chain assembly complex (LUBAC) generates heterotypic ubiquitin chains eLife Rodriguez Carvajal A, Grishkovskaya I, Gomez Diaz C, Vogel A, Sonn-Segev A, Kushwah MS, Schodl K, Deszcz L, Orban-Nemeth Z, Sakamoto S, Mechtler K, Kukura P, Clausen T, Haselbach D, Ikeda F Go to publication →
  • 2021 Identification and selectivity profiling of small-molecule degraders via multi-omics approaches Cell Chemical Biology Scholes NS, Mayor-Ruiz C, Winter GE Go to publication →
  • 2021 Cellular control of protein turnover via modification of the amino terminus International Journal of Molecular Sciences Winter N, Novatchkova M and Bachmair A Go to publication →
  • 2021 Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation Cell Reports Lobato-Gil S, Heidelberger JB, Maghames C, Bailly A, Brunello L, Rodriguez MS, Beli P*, Xirodimas DP* (*co-corresponding authors) Go to publication →
  • 2020 EXO70D isoforms mediate selective autophagic degradation of type-A ARR proteins to regulate cytokinin sensitivity Proc. Natl. Acad. Sci. USA Acheampong AK, Shanks C, Cheng CY, Schaller GE, Dagdas Y, Kieber JJ Go to publication →
  • 2020 Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation Science Sawa-Makarska J, Baumann V, Coudevylle N, von Bülow S, Nogellova V, Abert C, Schuschnig M, Graef M, Hummer G, Martens S Go to publication →
  • 2020 A cross-kingdom conserved ER-phagy receptor maintains endoplasmic reticulum homeostasis during stress eLife Stephani M, Picchianti L, Gajic A, Beveridge R, Skarwan R, Sanchez de Medina Hernandez V, Mohseni A, Clavel M, Zeng Y, Naumann C, Matuszkiewicz M, Turco E, Loefke C, Li B, Durnberger G, Schutzbier M, Chen HT, Abdrakhmanov A, Savova A, Chia KS, Djamei A, Schaffner I, Abel S, Jiang L, Mechtler K, Ikeda F, Martens S, Clausen T, Dagdas Y Go to publication →
  • 2020 Rational discovery of molecular glue degraders via scalable chemical profiling Nature Chemical Biology Mayor-Ruiz C, Bauer S, Brand M, Kozicka Z, Siklos M, Imrichova H, Kaltheuner IH, Hahn E, Seiler K, Koren A, Petzold G, Fellner M, Bock C, Müller AC, Zuber J, Geyer M, Thomä NH, Kubicek S, Winter GE Go to publication →
  • 2020 Native Mass Spectrometry Can Effectively Predict PROTAC Efficacy ACS Central Science Beveridge R, Kessler D, Rumpel K, Ettmayer P, Meinhart A, Clausen T Go to publication →
  • 2020 Moyamoya disease factor RNF213 is a giant E3 ligase with a dynein-like core and a distinct ubiquitin-transfer mechanism eLife Ahel J, Lehner A, Vogel A, Schleiffer A, Meinhart A, Haselbach D, Clausen T Go to publication →
  • 2020 Quantifying the heterogeneity of macromolecular machines by mass photometry Nature Communications Sonn-Segev A, Belacic K, Bodrug T, Young G, VanDerLindern R, Schulman BA, Schimpf J, Friedrich T, Dip PV, Schwartz TU, Bauer B, Peters JM, Struwe WB, Benesch JLP, Brown NG*, Haselbach D*, Kukura P* (*co-corresponding authors) Go to publication →
  • 2020 Selective Mediator dependence of cell-type-specifying transcription Nature Genetics Jaeger MG, Schwalb B, Mackowiak SD, Velychko T, Hanzl A, Imrichova H, Brand M, Agerer B, Chorn S, Nabet B, Ferguson FM, Müller AC, Bergthaler A, Gray NS, Bradner JE, Bock C, Hnisz D, Cramer P, Winter GE Go to publication →
  • 2020 A PI3K-WIPI2 positive feedback loop allosterically activates LC3 lipidation in autophagy Journal of Cell Biology Fracchiolla D, Chang C, Hurley JH, Martens S Go to publication →
  • 2020 Phase separation directs ubiquitination of gene-body nucleosomes Nature Gallego LD, Schneider M, Mittal C, Romanauska A, Gudino Carrillo RM, Schubert T, Pugh BF, Köhler A Go to publication →
  • 2020 Highly Precise Protein Semisynthesis through Ligation–Desulfurization Chemistry in Combination with Phenacyl Protection of Native Cysteines Methods in Molecular Biology Mukherjee S, Matveenko M, Becker CFW Go to publication →
  • 2020 Autophagy mediates temporary reprogramming and dedifferentiation in plant somatic cells EMBO Journal Rodriguez E, Chevalier J, Olsen J, Ansbøl J, Kapousidou V, Zuo Z, Svenning S, Loefke C, Koemeda S, Drozdowskyj PS, Jez J, Durnberger G, Kuenzl F, Schutzbier M, Mechtler K, Ebstrup EN, Lolle S, Dagdas Y*, Petersen M* Go to publication →
  • 2020 Targeted protein degradation: current and future challenges Current Opinion in Chemical Biology Hanzl A. Winter GE Go to publication →
  • 2019 Quiescence of Adult Mammalian Neural Stem Cells: A Highly Regulated Rest Neuron Urbán N, Blomfield IM, Guillemot F Go to publication →
  • 2019 The RNA-binding ubiquitin ligase MKRN1 functions in ribosome-associated quality control of poly(A) translation Genome Biolology Hildebrandt A, Brüggemann M, Rücklé C, Boerner S, Heidelberger JB, Busch A, Hänel H, Voigt A, Möckel MM, Ebersberger S, Scholz A, Dold A, Schmid T, Ebersberger I, Roignant JY, Zarnack K, König J, Beli P Go to publication →
  • 2019 Id4 promotes the elimination of the pro-activation factor Ascl1 to maintain quiescence of adult hippocampal stem cells eLife Blomfield IM, Rocamonde B, Masdeu MDM, Mulugeta E, Vaga S, van den Berg DL, Huillard E, Guillemot F, Urbán N Go to publication →
  • 2019 Labeling and Natural Post-Translational Modification of Peptides and Proteins via Chemoselective Pd-Catalyzed Prenylation of Cysteine Journal of American Chemical Society Schlatzer T, Kriegesmann J, Schröder H, Trobe M, Lembacher-Fadum C, Santner S, Kravchuk AV, Becker CFW, Breinbauer R Go to publication →
  • 2019 Protein engineering of a ubiquitin-variant inhibitor of APC/C identifies a cryptic K48 ubiquitin chain binding site Proc. Natl. Acad. Sci. USA Watson ER, Grace CRR, Zhang W, Miller DJ, Davidson IF, Prabu JR, Yu S, Bolhuis DL, Kulko ET, Vollrath R, Haselbach D, Stark H, Peters JM, Brown NG, Sidhu SS, Schulman BA Go to publication →
  • 2019 N-terminal β-strand underpins biochemical specialization of an ATG8 isoform PLoS Biology Zess EK, Jensen C, Cruz-Mireles N, De la Concepcion JC, Sklenar J, Stephani M, Imre R, Roitinger E, Hughes R, Belhaj K, Mechtler K, Menke FLH, Bozkurt T, Banfield MJ, Kamoun S, Maqbool A, Dagdas YF Go to publication →
  • 2019 Plasticity of the cullin-RING ligase repertoire shapes sensitivity to ligand-induced protein degradation Molecular Cell Mayor-Ruiz C, Jaeger MG, Bauer S, Brand M, Sin C, Hanzl A, Mueller AC, Menche J, Winter GE Go to publication →
  • 2019 A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52 Scientific Reports Hacker K, Benke S, Agerer B, Scinicariello S, Budroni V, Versteeg GA Go to publication →
  • 2019 MTHFD1 interaction with BRD4 links folate metabolism to transcriptional regulation Nature Genetics Sdelci S, Rendeiro AF, Rathert P, You W, Lin JG, Ringler A, Hofstätter G, Moll HP, Gürtl B, Farlik M, Schick S, Klepsch F, Oldach M, Buphamalai P, Schischlik F, Májek P, Parapatics K, Schmidl C, Schuster M, Penz T, Buckley DL, Hudecz O, Imre R, Wang SY, Maric HM, Kralovics R, Bennett KL, Müller AC, Mechtler K, Menche J, Bradner JE, Winter GE, Klavins K, Casanova E, Bock C, Zuber J, Kubicek S Go to publication →
  • 2019 FIP200 Claw Domain Binding to p62 Promotes Autophagosome Formation at Ubiquitin Condensates Molecular Cell Turco E, Witt M, Abert C, Bock-Bierbaum T, Su MY, Trapannone R, Sztacho M, Danieli A, Shi X, Zaffagnini G, Gamper A, Schuschnig M, Fracchiolla D, Bernklau D, Romanov J, Hartl M, Hurley JH, Daumke O, Martens S Go to publication →
  • 2019 Structure of McsB, a protein kinase for regulated arginine phosphorylation Nature Chemical Biology Suskiewicz MJ, Hajdusits B, Beveridge R, Heuck A, Vu LD, Kurzbauer R, Hauer K, Thoeny V, Rumpel K, Mechtler K, Meinhart A, Clausen T Go to publication →
  • 2019 Locus-Specific Knock-in of a degradable tag for target validation studies Methods in Molecular Biology Brand M, Winter GE Go to publication →
  • 2019 Homolog-Selective Degradation as a Strategy to Probe the Function of CDK6 in AML Cell Chemical Biology Brand M, Jiang B, Bauer S, Donovan KA, Liang Y, Wang ES, Nowak RP, Yuan JC, Zhang T, Kwiatkowski N, Müller AC, Fischer ES, Gray NS, Winter GE Go to publication →
  • 2019 The Scope, Functions, and Dynamics of Posttranslational Protein Modifications Annu. Rev. Plant Biol. Millar AH, Heazlewood JL, Giglione C, Holdsworth MJ, Bachmair A, Schulze WX Go to publication →
  • 2019 Identification and characterization of cancer vulnerabilities via targeted protein degradation Drug Discov. Today Technol. Mayor-Ruiz C, Winter GE Go to publication →
  • 2018 The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1 eLife Acosta-Alvear D, Karagöz GE, Fröhlich F, Li H, Walther TC, Walter P Go to publication →
  • 2019 Single Posttranslational Modifications in the Central Repeat Domains of Tau4 Impact its Aggregation and Tubulin Binding Angewandte Chemie International Ed. Ellmer D, Brehs M, Haj-Yahya M, Lashuel HA, Becker CFW Go to publication →
  • 2018 LZTR1 is a regulator of RAS ubiquitination and signaling Science Bigenzahn JW, Collu GM, Kartnig F, Pieraks M, Vladimer GI, Heinz LX, Sedlyarov V, Schischlik F, Fauster A, Rebsamen M, Parapatics K, Blomen VA, Müller AC, Winter GE, Kralovics R, Brummelkamp TR, Mlodzik M, Superti-Furga G Go to publication →
  • 2018 Native chemical ligation in protein synthesis and semi-synthesis Chem. Soc. Rev. Conibear AC, Watson EE, Payne RJ, Becker CFW Go to publication →
  • 2019 Distinct branches of the N-end rule pathway modulate the plant immune response New Phytologist Vicente J, Mendiondo GM, Pauwels J, Pastor V, Izquierdo Y, Naumann C, Movahedi M, Rooney D, Gibbs DJ, Smart K, Bachmair A, Gray JE, Dissmeyer N, Castresana C, Ray RV, Gevaert K, Holdsworth MJ Go to publication →
  • 2018 The inner nuclear membrane is a metabolically active territory that generates nuclear lipid droplets Cell Romanauska A, Köhler A Go to publication →
  • 2018 Host autophagy machinery is diverted to the pathogen interface to mediate focal defense responses against the Irish potato famine pathogen eLife Dagdas YF*, Pandey P*, Tumtas Y, Sanguankiattichai N, Belhaj K, Duggan C, Leary AY, Segretin ME, Contreras MP, Savage Z, Khandare VS, Kamoun S, Bozkurt TO Go to publication →
  • 2018 Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48 Nature Communications Velimezi G, Robinson-Garcia L, Muñoz-Martínez F, Wiegant WW, Ferreira da Silva J, Owusu M, Moder M, Wiedner M, Rosenthal SB, Fisch KM, Moffat J, Menche J, van Attikum H, Jackson SP, Loizou JI Go to publication →
  • 2018 Targetable BET proteins- and E2F1-dependent transcriptional program maintains the malignancy of glioblastoma Proc. Natl. Acad. Sci. USA Xu L, Chen Y, Mayakonda A, Koh L, Chong YK, Buckley DL, Sandanaraj E, Lim SW, Lin RY, Ke XY, Huang ML, Chen J, Sun W, Wang LZ, Goh BC, Dinh HQ, Kappei D, Winter GE, Ding LW, Ang BT, Berman BP, Bradner JE, Tang C, Koeffler HP Go to publication →
  • 2018 The dTAG system for immediate and target-specific protein degradation Nature Chemical Biology Nabet B, Roberts JM, Buckley DL, Paulk J, Dastjerdi S, Yang A, Leggett AL, Erb MA, Lawlor MA, Souza A, Scott TG, Vittori S, Perry JA, Qi J, Winter GE, Wong KK, Gray NS, Bradner JE Go to publication →
  • 2018 p38-MK2 signaling axis regulates RNA metabolism after UV-light-induced DNA damage Nature Communications Borisova ME, Voigt A, Tollenaere MAX, Sahu SK, Juretschke T, Kreim N, Mailand N, Choudhary C, Bekker-Jensen S, Akutsu M, Wagner SA, Beli P Go to publication →
  • 2018 Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands Nature Chemical Biology Gechijian LN, Buckley DL, Lawlor MA, Reyes JM, Paulk J, Ott CJ, Winter GE, Erb MA, Scott TG, Xu M, Seo HS, Dhe-Paganon S, Kwiatkowski NP, Perry JA, Qi J, Gray NS, Bradner JE Go to publication →
  • 2018 Proteomic profiling of VCP substrates links VCP to K6-linked ubiquitylation and c-Myc function EMBO Reports Heidelberger JB, Voigt A, Borisova ME, Petrosino G, Ruf S, Wagner SA, Beli P Go to publication →
  • 2018 Human tripartite motif protein 52 is required for cell context-dependent proliferation Oncotarget Benke S, Agerer B, Haas L, Stöger M, Lercher A, Gabler L, Kiss I, Scinicariello S, Berger W, Bergthaler A, Obenauf AC, Versteeg GA Go to publication →
  • 2018 UFD-2 is an adaptor-assisted E3 ligase targeting unfolded proteins Nature Communications Hellerschmied D, Roessler M, Lehner A, Gazda L, Stejskal K, Imre R, Mechtler K, Dammermann A, Clausen T Go to publication →
  • 2018 Translation Termination Factor GSPT1 Is a Phenotypically Relevant Off-Target of Heterobifunctional Phthalimide Degraders ACS Chem. Biol. Ishoey M, Chorn S, Singh N, Jaeger MG, Brand M, Paulk J, Bauer S, Erb MA, Parapatics K, Müller AC, Bennett KL, Ecker GF, Bradner JE, Winter GE Go to publication →
  • 2018 p62 filaments capture and present ubiquitinated cargos for autophagy EMBO Journal Zaffagnini G, Savova A, Danieli A, Romanov J, Tremel S, Ebner M, Peterbauer T, Sztacho M, Trapannone R, Tarafder AK, Sachse C, Martens S Go to publication →
  • 2018 SUMO chain formation relies on the amino-terminal region of SUMO conjugating enzyme and has dedicated substrates in plants Biochemical J. Tomanov, K., Nehlin, L., Ziba, I., Bachmair, A. Go to publication →
  • 2018 Acute Pharmacologic Degradation of a Stable Antigen Enhances Its Direct Presentation on MHC Class I Molecules Front. Immunol. Moser SC, Voerman JSA, Buckley DL, Winter GE, Schliehe C Go to publication →
  • 2018 Pharmacological perturbation of CDK9 using selective CDK9 inhibition or degradation Nat. Chem. Biol. Olson CM, Jiang B, Erb MA, Liang Y, Doctor ZM, Zhang Z, Zhang T, Kwiatkowski N, Boukhali M, Green JL, Haas W, Nomanbhoy T, Fischer ES, Young RA, Bradner JE, Winter GE, Gray NS Go to publication →
  • 2017 Repair of UV-Induced DNA Damage Independent of Nucleotide Excision Repair Is Masked by MUTYH Mol. Cell Mazouzi A, Battistini F, Moser SC, Ferreira da Silva, Wiedner M, Owusu M, Lardeau C-H, Ringler A, Weil B, Neesen J, Orozco M, Kubicek S, Loizou JI Go to publication →
  • 2017 Parallel genome-wide screens identify a synthetic viable interaction between the BLM helicase complex and Fanconi anemia Nat. Commun. Moder M, Velimezi G, Owusu M, Mazouzi A, Wiedner M, Ferreira da Silva J, Robinson-Garcia L, Schischlik F, Slavkovsky R, Kralovics R, Schuster M, Bock C, Ideker T, Jackson SP, Menche J, Loizou JI Go to publication →
  • 2017 An unfolded protein-induced conformational switch activates mammalian IRE1 eLife Karagöz GE, Acosta-Alvear D, Nguyen HT, Lee CP, Chu F, Walter P Go to publication →
  • 2017 MELK is not necessary for the proliferation of basal-like breast cancer cells eLife Huang HT, Seo HS, Zhang T, Wang Y, Jiang B, Li Q, Buckley DL, Nabet B, Roberts JM, Paulk J, Dastjerdi S, Winter GE, McLauchlan H, Moran J, Bradner JE, Eck MJ, Dhe-Paganon S, Zhao JJ, Gray NS Go to publication →
  • 2017 Long-range allosteric regulation of the human 26S proteasome by 20S proteasome-targeting cancer drugs Nat. Commun. Haselbach D , Schrader J, Lambrecht F, Henneberg F, Chari A, Stark H Go to publication →
  • 2017 Semisynthetic prion protein (PrP) variants carrying glycan mimics at position 181 and 197 do not form fibrils Chem. Sci. Araman C, Thompson RE, Wang S, Hackl S, Payne RJ, Becker CFW Go to publication →
  • 2017 BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment Mol. Cell Winter GE, Mayer A, Buckley DL, Erb MA, Roderick JE, Vittori S, Reyes JM, di Iulio J, Souza A, Ott CJ, Roberts JM, Zeid R, Scott TG, Paulk J, Lachance K, Olson CM, Dastjerdi S, Bauer S, Lin CY, Gray NS, Kelliher MA, Churchman LS, Bradner JE Go to publication →
  • 2017 Transcription control by the ENL YEATS domain in acute leukaemia Nature Erb MA, Scott TG, Li BE, Xie H, Paulk J, Seo HS, Souza A, Roberts JM, Dastjerdi S, Buckley DL, Sanjana NE, Shalem O, Nabet B, Zeid R, Offei-Addo NK, Dhe-Paganon S, Zhang F, Orkin SH, Winter GE, Bradner JE Go to publication →
  • 2016 Mechanism of cargo-directed Atg8 conjugation during selective autophagy eLife Fracchiolla D, Sawa-Makarska J, Zens B, Ruiter A, Zaffagnini G, Brezovich A, Romanov J, Runggatscher K, Kraft C, Zagrovic B, Martens S Go to publication →
  • 2016 Arginine phosphorylation marks proteins for degradation by a Clp protease Nature Trentini DB, Suskiewicz MJ, Heuck A, Kurzbauer R, Deszcz L, Mechtler K, Clausen T Go to publication →
  • 2016 Cryo-EM of Mitotic Checkpoint Complex-Bound APC/C Reveals Reciprocal and Conformational Regulation of Ubiquitin Ligation Mol. Cell Yamaguchi M, VanderLinden R, Weissmann F, Qiao R, Dube P, Brown NG, Haselbach D, Zhang W, Sidhu SS, Peters JM, Stark H, Schulman BA Go to publication →
  • 2016 Structural Basis of Host Autophagy-related Protein 8 (ATG8) Binding by the Irish Potato Famine Pathogen Effector Protein PexRD54 J. Biol. Chem. Maqbool A, Hughes RK, Dagdas YF, Tregidgo N, Zess E, Belhaj K, Round A, Bozkurt TO, Kamoun S, Banfield MJ Go to publication →
  • 2016 Return to quiescence of mouse neural stem cells by degradation of a proactivation protein Science Urbán N, van den Berg DL, Forget A, Andersen J, Demmers JA, Hunt C, Ayrault O, Guillemot F Go to publication →
  • 2016 Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C Cell Brown NG, VanderLinden R, Watson ER, Weissmann F, Ordureau Am Wu KP, Zhang W, Yu S, Mercedi PY, Harrison JS, Davidson IF, Qiao R, Lu Y, Dube P, Brunner MR, Grace CR, Miller DJ, Haselbach D, Jarvis MA, Yamaguchi M, Yanisheski D, Petzold G, Sidhu SS, Kuhlmann B, Kirschner MW, Harper JW, Peters JM, Stark H, Schulman BA Go to publication →
  • 2016 Mechanism of APC/CCDC20 activation by mitotic phosphorylation Proc. Natl. Acad. Sci. USA Qiao R, Weissmann F, Yamaguchi M, Brown NG, VanderLinden R, Imre R, Jarvis MA, Brunner MR, Davidson IF, Litos G, Haselbach D , Mechtler K, Stark H, Schulman BA, Peters JM Go to publication →
  • 2016 An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor eLife Dagdas YF*, Belhaj K*, Maqbool A, Chaparro-Garcia A, Pandey P, Petre B, Tabassum N, Cruz-Mireles N, Hughes RK, Sklenar J, Win J, Menke F, Findlay K, Banfield MJ, Kamoun S, Bozkurt TO Go to publication →
  • 2015 Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy eLife Wurzer B, Zaffagnini G, Fracchiolla D, Turco E, Abert C, Romanov J, Martens S Go to publication →
  • 2015 ProteoPlex: stability optimization of macromolecular complexes by sparse-matrix screening of chemical space Nat. Methods Chari A*, Haselbach D *, Kirves JM*, Ohmer J, Paknia E, Fischer N, Ganichkin O,Möller V, Frye JJ, Petzold G, Jarvis M, Tietzel M, Grimm C, Peters JM, Schulman BA, Tittmann K, Markl J, Fischer U, Stark H. (*equal contribution) Go to publication →
  • 2015 DRUG DEVELOPMENT. Phthalimide conjugation as a strategy for in vivo target protein degradation Science Winter GE, Buckley DL, Paulk J, Roberts JM, Souza A, Dhe-Paganon S, Bradner JE Go to publication →
  • 2014 The eukaryotic N-end rule pathway: conserved mechanisms and diverse functions Trends in Cell Biol. Gibbs DJ, Bacardit J, Bachmair A, Holdsworth MJ Go to publication →
  • 2014 Cargo binding to Atg19 unmasks additional Atg8 binding sites to mediate membrane–cargo apposition during selective autophagy Nat. Cell Biol. Sawa-Makarska J, Abert C, Romanov J, Zens B, Ibiricu I, Martens S Go to publication →
  • 2014 Hsp90-Tau complex reveals molecular basis for specificity in chaperone action Cell Karagöz GE, Duarte AM, Akoury E, Ippel H, Biernat J, Morán Luengo T, Radli M, Didenko T, Nordhues BA, Veprintsev DB, Dickey CA, Mandelkow E, Zweckstetter M, Boelens R, Madl T, Rüdiger SG Go to publication →
  • 2014 Nitric oxide sensing in plants is mediated by proteolytic control of group VII ERF transcription factors Mol. Cell Gibbs DJ, Md Isa N, Movahedi M, Lozano-Juste J, Mendiondo GM, Berckhan S, Marín-de la Rosa N, Vicente Conde J, Sousa Correia C, Pearce SP, Bassel GW, Hamali B, Talloji P, Tomé DF, Coego A, Beynon J, Alabadí D, Bachmair A, León J, Gray JE, Theodoulou FL, Holdsworth MJ Go to publication →