February 10, 2021

Definition of the NEDD8 Proteome in Homeostatic and Proteotoxic Stress conditions reveals a role for hybrid NEDD8-SUMO-2 chains in nuclear Protein Quality Control

New work published in Cell Reports, from the team of Dimitris Xirodima (CRBM, University of Montpellier) and Petra Beli (IMB), Mainz) uncovers a diverse proteome for NEDDylation.

To elucidate biological processes controlled by NEDDylation, the identification of modification sites for ubiquitin/Ubls is of critical importance. In this study Lobato-Gil et al.show the proteome-wide identification of NEDD8 sites defining distinct NEDD8 proteomes for canonical and atypical NEDDylation and diverse homo- and hybrid NEDD8 polymere formation. This results in the accumulation of NEDD8-SUMO-2 chains in nucleolus-related inclusions upon proteolytic stress.


This diverse proteome for NEDDylation is consistent with the concept of extensive cross-talk between ubiquitin and UBls under proteotoxic stress conditions.



Lobato-Gil S, Heidelberger JB, Maghames C, Bailly A, Brunello L, Rodriguez MS, Beli P*, Xirodimas DP* (* co-corresponding authors).


Proteome-wide identification of NEDD8 modification sites reveals distinct proteomes for canonical and atypical NEDDylation

Cell Reports. January 19, 2021


(Illustration: Graphical Abstract https://doi.org/10.1016/j.celrep.2020.108635)